Antibacterial effects of different low-level doses of double antibiotic paste on multispecies biofilms in regenerative endodontics: A confocal laser microscopy study

Şükriye Türkoğlu Kayacı(1), Seda Keleştemur(2), Zeynep Solmazgül Yazıcı(3), Nurver Ülger(4)
(1) University of Health Sciences, Hamidiye Faculty of Dentistry, Department of Pediatric Dentistry, Istanbul, Türkiye,
(2) City University of New York, City College of New York, Department of Chemistry and Biochemistry, New York City, NY, United States,
(3) University of Health Sciences, Hamidiye Faculty of Dentistry, Department of Pediatric Dentistry, Istanbul, Türkiye,
(4) Marmara University, School of Medicine, Department of Microbiology, Istanbul, Türkiye

Abstract

Aim: This research aimed to assess the comparative efficacy of various concentrations of double antibiotic paste used on multispecies biofilms in regenerative endodontic procedures.


Methodology: Seventy caries-free human teeth with a single root that were extracted for orthodontic purposes were gathered. A total of 900 μL of bacterial suspension was prepared, comprising three bacterial species: E. faecalis (ATCC 43146), A. naeslundii (ATCC 10556), and S. sanguinis (ATCC 29212). Six experimental groups (n=10/group) were randomly created with the infected dentin slices: 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/mlof double antibiotic paste and a control group. Confocal laser scanning microscopy (CLSM) analysis was conducted to evaluate the antimicrobial activity. Two samples from each group were analyzed with a scanning electron microscope. The live/dead bacteria percentages were analyzed via Kruskal–Wallis test. The tests were conducted with a significance level of 5%.


Results: Greater live/dead bacteria ratios were observed in the control group, and the differences compared to other groups were statistically significant. Additionally, the numbers of live/dead bacteria observed after the 0.5 mg/mL and 1 mg/mL DAP doses were applied were significantly higher compared to the 10 mg/mL DAP concentration. All DAP doses exhibited some degree of antibiofilm activity; however, 5 mg/mL and 10 mg/mL were the most effective.


Conclusion: In regenerative endodontics, the 5 mg/mL and 10 mg/mL doses of DAP demonstrated more effective activity against multispecies biofilms than lower doses.

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Authors

Şükriye Türkoğlu Kayacı
sukriye.turkoglu@sbu.edu.tr (Primary Contact)
Seda Keleştemur
Zeynep Solmazgül Yazıcı
Nurver Ülger
Türkoğlu Kayacı, Şükriye, Keleştemur, S., Solmazgül Yazıcı, Z., & Ülger, N. (2024). Antibacterial effects of different low-level doses of double antibiotic paste on multispecies biofilms in regenerative endodontics: A confocal laser microscopy study. International Dental Research. https://doi.org/10.5577/intdentres.539

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How to Cite

Türkoğlu Kayacı, Şükriye, Keleştemur, S., Solmazgül Yazıcı, Z., & Ülger, N. (2024). Antibacterial effects of different low-level doses of double antibiotic paste on multispecies biofilms in regenerative endodontics: A confocal laser microscopy study. International Dental Research. https://doi.org/10.5577/intdentres.539
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